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Expression of Stipa purpurea SpCIPK26 in Arabidopsis thaliana Enhances Salt and Drought Tolerance and Regulates Abscisic Acid Signaling
Zhou, YL (Zhou, Yanli)1,2,3; Sun, XD (Sun, Xudong)1,2,4; Yang, YQ (Yang, Yunqiang)1,2,4; Li, X (Li, Xiong)1,2,4; Cheng, Y (Cheng, Ying)5; Yang, YP (Yang, Yongping)1,2,3,4; Yang, YP
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
2016
Volume17Issue:6Pages:966
DOI10.3390/ijms17060966
AbstractStipa purpurea (S. purpurea) is the dominant plant species in the alpine steppe of the Qinghai-Tibet Plateau, China. It is highly resistant to cold and drought conditions. However, the underlying mechanisms regulating the stress tolerance are unknown. In this study, a CIPK gene from S. purpurea (SpCIPK26) was isolated. The SpCIPK26 coding region consisted of 1392 bp that encoded 464 amino acids. The protein has a highly conserved catalytic structure and regulatory domain. The expression of SpCIPK26 was induced by drought and salt stress. SpCIPK26 overexpression in Arabidopsis thaliana (A. thaliana) plants provided increased tolerance to drought and salt stress in an abscisic acid (ABA)-dependent manner. Compared with wild-type A. thaliana plants, SpCIPK26-overexpressing plants had higher survival rates, water potentials, and photosynthetic efficiency (Fv/Fm), as well as lower levels of reactive oxygen species (ROS) following exposure to drought and salt stress. Gene expression analyses indicated stress-inducible genes (RD29A, RD29B, and ABF2) and a ROS-scavenger gene (CAT1) were upregulated in SpCIPK26-overexpressing plants after stress treatments. All of these marker genes are associated with ABA-responsive cis-acting elements. Additionally, the similarities in the gene expression patterns following ABA, mannitol, and NaCl treatments suggest SpCIPK26 has an important role during plant responses to drought and salt stress and in regulating ABA signaling.
Subject Area植物学
WOS IDWOS:000378799300178
Language英语
Indexed BySCI
KeywordProtein-kinases Stress Tolerance Stipa-purpurea Abiotic Stress Electrolyte Leakage Gene-expression Calcium Sensors Osmotic-stress Plant-response Water-stress
Cooperation Status国内
Department生态
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.itpcas.ac.cn/handle/131C11/7683
Collection图书馆
Corresponding AuthorYang, YP
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650201, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Plant Germplasm & Genom Ctr, Kunming 650201, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Kunming Inst Bot, Inst Tibetan Plateau Res, Kunming 650201, Peoples R China
5.Yunnan Agr Univ, Coll Hort & Landscape, Kunming 650201, Peoples R China
Recommended Citation
GB/T 7714
Zhou, YL ,Sun, XD ,Yang, YQ ,et al. Expression of Stipa purpurea SpCIPK26 in Arabidopsis thaliana Enhances Salt and Drought Tolerance and Regulates Abscisic Acid Signaling[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2016,17(6):966.
APA Zhou, YL .,Sun, XD .,Yang, YQ .,Li, X .,Cheng, Y .,...&Yang, YP.(2016).Expression of Stipa purpurea SpCIPK26 in Arabidopsis thaliana Enhances Salt and Drought Tolerance and Regulates Abscisic Acid Signaling.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,17(6),966.
MLA Zhou, YL ,et al."Expression of Stipa purpurea SpCIPK26 in Arabidopsis thaliana Enhances Salt and Drought Tolerance and Regulates Abscisic Acid Signaling".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 17.6(2016):966.
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