ITPCAS OpenIR  > 图书馆
Application of orthogonal design to the extraction and HPLC analysis of sedimentary pigments from lakes of the Tibetan Plateau
Liang, J (Liang Jie)1,2; Li, D (Li Dong)3,4,5,6; Wang, MD (Wang MingDa)1,2; Zhang, XY (Zhang XinYu)7,8; Hou, JZ (Hou JuZhi)1; Hou, JZ
Source PublicationSCIENCE CHINA-EARTH SCIENCES
2016
Volume59Issue:6Pages:1195-1205
DOI10.1007/s11430-015-5240-1
AbstractSedimentary pigments are useful proxy indicators of phytoplankton biomass, community structure, primary productivity and human influence in lacustrine and oceanic ecosystems. Pigments are relatively labile due to their complex chemical structures, which makes the extraction and analysis of sedimentary pigments challenging. In addition, it is important to select appropriate methods to study sedimentary pigments in regions which lack previous investigations. In this study, we adopted the L-9(3(4)) orthogonal design to develop methods of extraction and HPLC (high performance liquid chromatography) analysis of sedimentary pigments at two lakes on the Tibetan Plateau: meromictic lake-Dagze Co and dimictic lake-Jiang Co. The orthogonal design comprises 9 combinations of various parameters for extraction and HPLC analysis. The results show that the type and volume of solvent are the most important factors for pigment extraction, and the mobile phase and column selection are the most important for HPLC analysis. For the study sites, we found that the best methods to extract sedimentary pigments are as follows: the use of a mixture of acetone:methanol:water (80:15:5, v:v:v) as the extraction solvent; solvent/ sample ratio of 10 mL/g; sonication for 30 s and standing extraction for 6 h. The best methods for HPLC analysis are as follows: Zorbax Eclipse plus C8 column with mobile phase A, methanol:acetonitrile:0.25 M aqueous pyridine (50:25:25, v:v:v) and mobile phase B, methanol: acetonitrile: acetone (20:60:20, v:v:v); pH of mobile phase A adjusted to 6 with acetic acid; and HPLC column temperature maintained at 40A degrees C. The study provides an experimental basis for future investigations of past changes in primary productivity and the response of lake ecosystems to climate change and human activities on the Tibetan Plateau.
Subject Area自然地理学
WOS IDWOS:000376675400007
Language英语
Indexed BySCI
KeywordLiquid-chromatography Marine-phytoplankton Alpine Lakes Chlorophylls Carotenoids Temperature Column Classification Productivity Abundance
Cooperation Status国内
Department环境
SubtypeArticle
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.itpcas.ac.cn/handle/131C11/7691
Collection图书馆
Corresponding AuthorHou, JZ
Affiliation1.Chinese Acad Sci, Inst Tibetan Plateau Res, Key Lab Tibetan Environm Changes & Land Surface P, Beijing 100101, Peoples R China
2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China
3.Minist Educ, Key Lab Marine Chem Theory & Technol, Qingdao 266100, Peoples R China
4.Ocean Univ China, Coll Chem & Chem Engn, Qingdao 266100, Peoples R China
5.State Ocean Adm, Inst Oceanog 2, Hangzhou 310012, Zhejiang, Peoples R China
6.State Ocean Adm, Key Lab Marine Ecosyst & Biogeochem, Hangzhou 310012, Zhejiang, Peoples R China
7.Peking Univ, MOE Key Lab Earth Surface Proc, Beijing 100871, Peoples R China
8.Peking Univ, Coll Urban & Environm Sci, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Liang, J ,Li, D ,Wang, MD ,et al. Application of orthogonal design to the extraction and HPLC analysis of sedimentary pigments from lakes of the Tibetan Plateau[J]. SCIENCE CHINA-EARTH SCIENCES,2016,59(6):1195-1205.
APA Liang, J ,Li, D ,Wang, MD ,Zhang, XY ,Hou, JZ ,&Hou, JZ.(2016).Application of orthogonal design to the extraction and HPLC analysis of sedimentary pigments from lakes of the Tibetan Plateau.SCIENCE CHINA-EARTH SCIENCES,59(6),1195-1205.
MLA Liang, J ,et al."Application of orthogonal design to the extraction and HPLC analysis of sedimentary pigments from lakes of the Tibetan Plateau".SCIENCE CHINA-EARTH SCIENCES 59.6(2016):1195-1205.
Files in This Item:
File Name/Size DocType Version Access License
V.59(6) 1195-1205 20(732KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Liang, J (Liang Jie)]'s Articles
[Li, D (Li Dong)]'s Articles
[Wang, MD (Wang MingDa)]'s Articles
Baidu academic
Similar articles in Baidu academic
[Liang, J (Liang Jie)]'s Articles
[Li, D (Li Dong)]'s Articles
[Wang, MD (Wang MingDa)]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Liang, J (Liang Jie)]'s Articles
[Li, D (Li Dong)]'s Articles
[Wang, MD (Wang MingDa)]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: V.59(6) 1195-1205 2016.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.