| Application of orthogonal design to the extraction and HPLC analysis of sedimentary pigments from lakes of the Tibetan Plateau | |
| Liang, J (Liang Jie)1,2; Li, D (Li Dong)3,4,5,6; Wang, MD (Wang MingDa)1,2; Zhang, XY (Zhang XinYu)7,8; Hou, JZ (Hou JuZhi)1; Hou, JZ | |
| Source Publication | SCIENCE CHINA-EARTH SCIENCES
 |
| 2016 | |
| Volume | 59Issue:6Pages:1195-1205 |
| DOI | 10.1007/s11430-015-5240-1 |
| Abstract | Sedimentary pigments are useful proxy indicators of phytoplankton biomass, community structure, primary productivity and human influence in lacustrine and oceanic ecosystems. Pigments are relatively labile due to their complex chemical structures, which makes the extraction and analysis of sedimentary pigments challenging. In addition, it is important to select appropriate methods to study sedimentary pigments in regions which lack previous investigations. In this study, we adopted the L-9(3(4)) orthogonal design to develop methods of extraction and HPLC (high performance liquid chromatography) analysis of sedimentary pigments at two lakes on the Tibetan Plateau: meromictic lake-Dagze Co and dimictic lake-Jiang Co. The orthogonal design comprises 9 combinations of various parameters for extraction and HPLC analysis. The results show that the type and volume of solvent are the most important factors for pigment extraction, and the mobile phase and column selection are the most important for HPLC analysis. For the study sites, we found that the best methods to extract sedimentary pigments are as follows: the use of a mixture of acetone:methanol:water (80:15:5, v:v:v) as the extraction solvent; solvent/ sample ratio of 10 mL/g; sonication for 30 s and standing extraction for 6 h. The best methods for HPLC analysis are as follows: Zorbax Eclipse plus C8 column with mobile phase A, methanol:acetonitrile:0.25 M aqueous pyridine (50:25:25, v:v:v) and mobile phase B, methanol: acetonitrile: acetone (20:60:20, v:v:v); pH of mobile phase A adjusted to 6 with acetic acid; and HPLC column temperature maintained at 40A degrees C. The study provides an experimental basis for future investigations of past changes in primary productivity and the response of lake ecosystems to climate change and human activities on the Tibetan Plateau. |
| Subject Area | 自然地理学 |
| WOS ID | WOS:000376675400007 |
| Language | 英语 |
| Indexed By | SCI |
| Keyword | Liquid-chromatography Marine-phytoplankton Alpine Lakes Chlorophylls Carotenoids Temperature Column Classification Productivity Abundance |
| Cooperation Status | 国内 |
| Department | 环境 |
| Subtype | Article |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.itpcas.ac.cn/handle/131C11/7691 |
| Collection | 图书馆 |
| Corresponding Author | Hou, JZ |
| Affiliation | 1.Chinese Acad Sci, Inst Tibetan Plateau Res, Key Lab Tibetan Environm Changes & Land Surface P, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 3.Minist Educ, Key Lab Marine Chem Theory & Technol, Qingdao 266100, Peoples R China 4.Ocean Univ China, Coll Chem & Chem Engn, Qingdao 266100, Peoples R China 5.State Ocean Adm, Inst Oceanog 2, Hangzhou 310012, Zhejiang, Peoples R China 6.State Ocean Adm, Key Lab Marine Ecosyst & Biogeochem, Hangzhou 310012, Zhejiang, Peoples R China 7.Peking Univ, MOE Key Lab Earth Surface Proc, Beijing 100871, Peoples R China 8.Peking Univ, Coll Urban & Environm Sci, Beijing 100871, Peoples R China |
| Recommended Citation GB/T 7714 | Liang, J ,Li, D ,Wang, MD ,et al. Application of orthogonal design to the extraction and HPLC analysis of sedimentary pigments from lakes of the Tibetan Plateau[J]. SCIENCE CHINA-EARTH SCIENCES,2016,59(6):1195-1205. |
| APA | Liang, J ,Li, D ,Wang, MD ,Zhang, XY ,Hou, JZ ,&Hou, JZ.(2016).Application of orthogonal design to the extraction and HPLC analysis of sedimentary pigments from lakes of the Tibetan Plateau.SCIENCE CHINA-EARTH SCIENCES,59(6),1195-1205. |
| MLA | Liang, J ,et al."Application of orthogonal design to the extraction and HPLC analysis of sedimentary pigments from lakes of the Tibetan Plateau".SCIENCE CHINA-EARTH SCIENCES 59.6(2016):1195-1205. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| V.59(6) 1195-1205 20（732KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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