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The accretionary complex of the Yarlung Zangbo Suture Zone (YZSZ) in southern Tibet records the subduction-accretion process of Neo-Tethys oceanic lithosphere. We report field observations, petrographic analysis, detrital zircon U-Pb geochronology, and Hf isotope data of one sedimentary-matrix melange unit of YZSZ in Sangsang area, central southern Tibet. This melange unit is the northernmost, and presumably the oldest one within the YZSZ accretionary complex. The sandstone matrix and blocks are rich in volcanic detritus, linking the main provenance to a juvenile magmatic arc. U-Pb-Hf analysis of detrital zircons from the sandstones reveals a unimodal age population of similar to 186-121 Ma with high positive epsilon Hf(t) values of +6.67 to +14.58, consistent with derivation from Gangdese Arc on southern margin of Lhasa terrane. The maximum depositional age of the melange unit is limited to be 149-134 Ma by averaging the youngest detrital zircon ages. The sandstones of this melange unit probably deposited in the trench basin within a few million years of early accretion driven by the Neo-Tethys oceanic subduction. The development of arc-trench system verified the initiation of subduction of Neo-Tethys oceanic lithosphere in Early Jurassic. Our results and integration of previous studies on subduction-accretion process of Neo-Tethys subduction indicate that the accretionary complex in Sangsang area documented at least two stages of accretion processes, stage 1 in earliest Cretaceous and stage 2 in Late Cretaceous, separated by a period of forearc extension forming the Yarlung Zangbo Ophiolites. These two stages of accretion correspond to growth and erosion of Gangdese Arc and crustal shortening in southern Eurasian margin.