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Molecular cloning and characterization of the glutathione reductase gene from Stipa purpurea
Wang, QL (Wang, Qiuli)1,2,3,4; Pu, YA (Pu, Yanan)1,2,3,5; Yang, DN (Yang, Danni)1,2,3,5; Yin, X (Yin, Xin)1,2,3,5; He, ZR (He, Zhaorong)4; Yang, YQ (Yang, Yunqiang)1,2,3; Yang, YP (Yang, Yongping)1,2,3
Source PublicationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2018-01-08
Volume495Issue:2Pages:1851-1857
DOI10.1016/j.bbrc.2017.12.054
AbstractReactive oxygen species (ROS) are a key factor in abiotic stresses; excess ROS is harmful to plants. Glutathione reductase (GR) plays an important role in scavenging ROS in plants. Here, a GR gene, named SpGR, was cloned from Stipa purpurea and characterized. The full-length open reading frame was 1497 bp, encoding 498 amino acids. Subcellular localization analysis indicated that SpGR was localized to both the plasma membrane and nucleus. The expression of SpGR was induced by cold, salt, and drought stresses. Functional analysis indicated that ectopic expression of SpGR in Arabidopsis thaliana resulted in greater tolerance to salt stress than that of wild-type plants, but no difference under cold or drought treatments. The results of GR activity and GSSG and GSH content analyses suggested that, under salt stress, transgenic plants produced more GR to reduce GSSG to GSH for scavenging ROS than wild-type plants. Therefore, SpGR may be a candidate gene for plants to resist abiotic stress. (C) 2017 Elsevier Inc. All rights reserved.
WOS IDWOS:000424732700042
Language英语
Indexed BySCIE
KeywordEXPRESSION ANALYSIS ANTIOXIDANT SYSTEMS HYDROGEN-PEROXIDE OXIDATIVE STRESS SALT STRESS OXYGEN TOLERANCE ASCORBATE RESPONSES ENZYMES
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
Cooperation Status国内
ISSN0006-291X
Department高寒生态重点实验室
PublisherACADEMIC PRESS INC ELSEVIER SCIENCE
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.itpcas.ac.cn/handle/131C11/8763
Collection图书馆
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650204, Yunnan, Peoples R China;
2.Chinese Acad Sci, Kunming Inst Bot, Plant Germplasm & Genom Ctr, Kunming 650201, Yunnan, Peoples R China;
3.Chinese Acad Sci, Kunming Inst Bot, Inst Tibetan Plateau Res Kunming, Kunming 650201, Yunnan, Peoples R China;
4.Yunnan Univ, Sch Life Sci, Kunming 650091, Yunnan, Peoples R China;
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China.
Recommended Citation
GB/T 7714
Wang, QL ,Pu, YA ,Yang, DN ,et al. Molecular cloning and characterization of the glutathione reductase gene from Stipa purpurea[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2018,495(2):1851-1857.
APA Wang, QL .,Pu, YA .,Yang, DN .,Yin, X .,He, ZR .,...&Yang, YP .(2018).Molecular cloning and characterization of the glutathione reductase gene from Stipa purpurea.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,495(2),1851-1857.
MLA Wang, QL ,et al."Molecular cloning and characterization of the glutathione reductase gene from Stipa purpurea".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 495.2(2018):1851-1857.
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