A Splice Variant of BrrWSD1 in Turnip (Brassica rapa var. rapa) and Its Possible Role in Wax Ester Synthesis under Drought Stress | |
Yang, YQ (Yang, Yunqiang)1,2,3; Pu, YA (Pu, Yanan)1,2,3; Yin, X (Yin, Xin)1,2,3,4; Du, JC (Du, Jiancan)1; Zhou, ZL (Zhou, Zhili)1,2,3; Yang, DN (Yang, Danni)1,2,3,4); Sun, XD (Sun, Xudong)1,2,3; Sun, H (Sun, Hang)1,2,3; Yang, YP (Yang, Yongping)1,2,3 | |
Source Publication | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
![]() |
2019 | |
Volume | 67Issue:40Pages:11077-11088 |
DOI | 10.1021/acs.jafc.9b04069 |
Abstract | Cuticular wax accumulation in plants contributes to drought tolerance. Here, we compared the drought levels on two varieties with different genotypes in turnip (Brassica rapa var. rapa) and found that the drought tolerance was higher in the waxy KTRG-B48a than in the wax-free KTRG-B48b. A combination of PacBio and Illumina sequencing analyses revealed that differential transcripts were mainly enriched in the wax synthesis pathway, and a splice variant (BrrWSD1-X2) was identified in the waxy KTRG-B48a. BrrWSD1-X2 had a stronger ability to synthesize wax esters than BrrWSD1-X1 using heterologous expression in yeast (Saccharomyces cerevisiae) mutant H1246a. Then, we speculated that the T to C transversion of the third intron and the higher number of TA repeats in the third intron of BrrWSD1 DNA in the waxy KTRG-B48a may result in a lower efficiency of splicing recognition of the third intron, resulting in the emergence of BrrWSD1-X2 in waxy varieties. |
Subject Area | Agriculture |
WOS ID | WOS:000490354700009 |
Language | 英语 |
Indexed By | SCI |
Keyword | Cuticular Wax Water-stress Biosynthesis Leaf Accumulation Alignment Alfalfa Protein Events Gene |
WOS Research Area | Agriculture ; Chemistry ; Food Science & Technology |
WOS Subject | Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology |
Cooperation Status | 国内 |
ISSN | 0021-8561 |
Department | 高寒生态重点实验室 |
URL | 查看原文 |
Publisher | AMER CHEMICAL SOC |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.itpcas.ac.cn/handle/131C11/9129 |
Collection | 图书馆 |
Corresponding Author | Yang, YP (Yang, Yongping) |
Affiliation | 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650204, Yunnan, Peoples R China; 2.Chinese Acad Sci, Kunming Inst Bot, Plant Germplasm & Genom Ctr, Kunming 650201, Yunnan, Peoples R China; 3.Chinese Acad Sci, Kunming Inst Bot, Inst Tibetan Plateau Res Kunming, Kunming 650201, Yunnan, Peoples R China; 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China. |
Recommended Citation GB/T 7714 | Yang, YQ ,Pu, YA ,Yin, X ,et al. A Splice Variant of BrrWSD1 in Turnip (Brassica rapa var. rapa) and Its Possible Role in Wax Ester Synthesis under Drought Stress[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2019,67(40):11077-11088. |
APA | Yang, YQ .,Pu, YA .,Yin, X .,Du, JC .,Zhou, ZL .,...&Yang, YP .(2019).A Splice Variant of BrrWSD1 in Turnip (Brassica rapa var. rapa) and Its Possible Role in Wax Ester Synthesis under Drought Stress.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,67(40),11077-11088. |
MLA | Yang, YQ ,et al."A Splice Variant of BrrWSD1 in Turnip (Brassica rapa var. rapa) and Its Possible Role in Wax Ester Synthesis under Drought Stress".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 67.40(2019):11077-11088. |
Files in This Item: | ||||||
File Name/Size | DocType | Version | Access | License | ||
2019094.pdf(6017KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment