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Methane production in the oxygenated water column of a perennially ice-covered Antarctic lake
Li, W (Li, Wei)1; Dore, JE (Dore, John E.)1; Steigmeyer, AJ (Steigmeyer, August J.)2; Cho, YJ (Cho, Yong-Joon)3; Kim, OS (Kim, Ok-Sun)3; Liu, YQ (Liu, Yongqin)4; Morgan-Kiss, RM (Morgan-Kiss, Rachael M.)5; Skidmore, ML (Skidmore, Mark L.)2; Priscu, JC (Priscu, John C.)1
Source PublicationLIMNOLOGY AND OCEANOGRAPHY
2019
Volume65Issue:1Pages:143-156
DOI10.1002/lno.11257
Abstract

Aerobic methane production in aquatic ecosystems impacts the global atmospheric budget of methane, but the extent, mechanism, and taxa responsible for producing this greenhouse gas are not fully understood. Lake Bonney (LB), a perennially ice-covered Antarctic lake, has cold hypersaline waters underlying an oxygenated freshwater layer. We present temporal methane concentration profiles in LB indicating methane production in the oxygenated (> 200% air saturation) water. Experiments amended with methylphosphonate (MPn) yielded methane generation, suggesting in situ methanogenesis via the carbon-phosphorus (C-P) lyase pathway. Enrichment cultures from the lake were used to isolate five bacterial strains capable of generating methane when supplied with MPn as the sole P source. Based on 16S rRNA gene sequencing, the isolates belong to the Proteobacteria (closely related to Marinomonas, Hoeflea, and Marinobacter genera) and Bacteroidetes (Algoriphagus genus). 16S rRNA metagenomic sequencing confirms the presence of these taxa in LB. None of the isolated species were reported to be capable to produce methane. In addition, orthologs of the phosphoenolpyruvate mutase gene (PepM) and methylphosphonate synthase (MPnS), enzymes involved in phosphonate and MPn biosynthesis, were widely spread in the LB shotgun metagenomic libraries; genes related to C-P lyase pathways (phn gene clusters) were also abundant. 16S rRNA and mcrA genes of anaerobic methanogens were absent in both 16S rRNA and metagenomics libraries. These data reveal that in situ aerobic biological methane production is likely a significant source of methane in LB.

Subject AreaMarine & Freshwater Biology
WOS IDWOS:000476977600001
Language英语
Indexed BySCI
KeywordMcmurdo Dry Valleys Atmospheric Methane Aerobic Production Taylor Glacier Blood Falls Sp-nov. Phosphorus Diversity Bacterial Methylphosphonate
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS SubjectLimnology ; Oceanography
Cooperation Status国际
ISSN0024-3590
Department环境变化与地表过程重点实验室
URL查看原文
PublisherWILEY
SubtypeArticle
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Document Type期刊论文
Identifierhttp://ir.itpcas.ac.cn/handle/131C11/9232
Collection图书馆
Corresponding AuthorPriscu, JC (Priscu, John C.)
Affiliation1.Montana State Univ, Dept Land Resources & Environm Sci, Bozeman, MT 59717 USA;
2.Montana State Univ, Dept Earth Sci, Bozeman, MT 59717 USA;
3.Korea Polar Res Inst, Div Polar Life Sci, Incheon, South Korea;
4.Chinese Acad Sci, Inst Tibetan Plateau Res, Beijing, Peoples R China;
5.Miami Univ, Dept Microbiol, Oxford, OH 45056 USA.
Recommended Citation
GB/T 7714
Li, W ,Dore, JE ,Steigmeyer, AJ ,et al. Methane production in the oxygenated water column of a perennially ice-covered Antarctic lake[J]. LIMNOLOGY AND OCEANOGRAPHY,2019,65(1):143-156.
APA Li, W .,Dore, JE .,Steigmeyer, AJ .,Cho, YJ .,Kim, OS .,...&Priscu, JC .(2019).Methane production in the oxygenated water column of a perennially ice-covered Antarctic lake.LIMNOLOGY AND OCEANOGRAPHY,65(1),143-156.
MLA Li, W ,et al."Methane production in the oxygenated water column of a perennially ice-covered Antarctic lake".LIMNOLOGY AND OCEANOGRAPHY 65.1(2019):143-156.
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