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学科主题: 生物科学::植物学
题名: Overexpression of SpCBL6, a calcineurin B-like protein of Stipa purpurea, enhanced cold tolerance and reduced drought tolerance in transgenic Arabidopsis
作者: Zhou, YL (Zhou, Yanli)1, 2, 3; Cheng, Y (Cheng, Ying)5; Yang, YQ (Yang, Yunqiang)1, 2, 4; Li, X (Li, Xiong)1, 2, 4; Supriyo, B (Supriyo, Basak)1, 2, 4; Sun, XD (Sun, Xudong)1, 2, 4; Yang, YP (Yang, Yongping)1, 2, 3, 4
通讯作者: Sun, XD ; Yang, YP
关键词: GENE-EXPRESSION ; ABIOTIC STRESS ; TRANSCRIPTION FACTORS ; CALCIUM SENSORS ; LOW-TEMPERATURE ; SALT TOLERANCE ; ABSCISIC-ACID ; PLANTS ; RESPONSES ; THALIANA
刊名: MOLECULAR BIOLOGY REPORTS
发表日期: 2016
DOI: 10.1007/s11033-016-4036-5
卷: 43, 期:9, 页:957-966
收录类别: SCI
合作性质: 国内
文章类型: Article
部门归属: 生态
英文摘要: The purpose of the present study was to characterize SpCBL6 (GenBank accession number: KT780442) from Stipa purpurea and elucidate the function of this protein in abiotic stress. The full-length cDNA of SpCBL6 was isolated from S. purpurea by rapid amplification of cDNA ends methods. Laser confocal microscopy was used to analyze the subcellular localization of SpCBL6. The constructs of 35S:GFP-SpCBL6 was used to transform wild-type (WT) Arabidopsis plants (ecotype Columbia-0) with the floral dip method. Quantitative reverse-transcription PCR (qRT-PCR), water potential, photosynthetic efficiency (F (v)/F (m)), and ion leakage was performed to investigate the role of SpCBL6 in abiotic stress. The open reading frame of SpCBL6 contains 681 bp nucleotides and encodes a 227-amino acid polypeptide. Phylogenetic analysis indicated that SpCBL6 showed the highest similarity with rice OsCBL6. SpCBL6 transcripts were induced by freezing and drought treatments. Subcellular localization analysis showed that SpCBL6 was located in membrane of protoplast. Overexpression of SpCBL6 in Arabidopsis thaliana demonstrated that the transgenic plants were more tolerant to cold treatment, but less tolerant to drought, compared with the plants. qRT-PCR analysis showed that the drought stress marker genes were inhibited in transgenic plants, whereas the cold stress marker genes were enhanced. Further analysis showed that SpCBL6-overexpressing plants showed enhanced water potential, photosynthetic efficiency (F (v)/F (m)), and reduced ion leakage compared with the wild-type after cold treatment. Collectively, these results indicate that SpCBL6, a new member of the CBL gene family isolated from S. purpurea, enhances cold tolerance and reduces drought tolerance in plants.
语种: 英语
WOS记录号: WOS:000382144400008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.itpcas.ac.cn/handle/131C11/7614
Appears in Collections:图书馆_期刊论文

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作者单位: 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650201, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Plant Germplasm & Genom Ctr, Germplasm Bank Wild Species, Kunming 650201, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Inst Tibetan Plateau Res, Kunming Inst Bot, Kunming 650201, Peoples R China
5.Yunnan Agr Univ, Coll Hort & Landscape, Kunming 650201, Peoples R China

Recommended Citation:
Zhou, YL ,Cheng, Y ,Yang, YQ ,et al. Overexpression of SpCBL6, a calcineurin B-like protein of Stipa purpurea, enhanced cold tolerance and reduced drought tolerance in transgenic Arabidopsis[J]. MOLECULAR BIOLOGY REPORTS,2016,43(9):957-966.
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文件名: V.43(9) 957-966 2016.pdf
格式: Adobe PDF
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