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Paleoaltimetric reconstructions of the southern Tibetan Plateau (TP) are crucial to understand the processes and mechanisms concomitant with the tectonic collision of the Indian and Eurasian continental plates. We measured the branched glycerol dialkyl glycerol tetraether (brGDGT) indices from 88 surface soils from the southern TP and found that alkaline soils exhibited lower MBT and MBT/CBT-derived MAT (mean annual temperature), while MBT/CBT-derived MAT exhibited a strong correlation with altitude (R-2=0.84; n=63) for all of the localities with neutral and acidic soils. Differently, we found that MBT5ME-derived MAT exhibited a strong relation with altitude even though a mixture of alkaline samples (R-2=0.72; n=83). As a result, two regional calibrations were developed, including a neutral and acidic soil calibration based on MBT/CBT-MAT and a calibration suitable for both acidic and alkaline soils based on MBT5ME-MAT for the southern TP. Based on this new soil calibration data set, and a recent global soil calibration data set founded on MBT5ME, we calculated paleo-MAT of approximate to 9.491.15 to 10.381.47 degrees C at approximate to 15 Ma, respectively, for the Namling Basin. Applying these soil calibrations, the paleoaltitudes of the Namling Basin can be calculated to have been between 4.360.41 km above sea level (asl) and 4.430.36 km asl at approximate to 15 Ma, respectively, similar to the paleoaltitudes of approximate to 4.7-5.4 km asl at approximate to 15 Ma inferred from the floral physiognomy of Namling Basin fossils and stable isotopes. These findings would suggest the applicability of MBT/CBT (and/or MBT5ME) indices as paleotopographic and paleotemperature proxies on the southern TP.