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A Gram-stain-positive, rod-shaped and motile bacterium with lateral flagellum, designated 13246-1(T) was isolated from an ice core, which was drilled from Hariqin Glacier on the Tibetan Plateau, PR China. It grew optimally at 20 degrees C, pH 7-8 and in the presence of 3% (w/v) NaCl. The major fatty acid of strain T3246-(T) was anteiso-C-15:0. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. MK-8 was the dominant isoprenoid quinone. The whole-cell sugars were rhamnose, xylose and mannose. The major cell-wall peptidoglycan was lysine. The genomic DNA G+C content of the strain was 71.4 mol%. Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain T3246-1(T) formed a lineage within the genus Haloactinobacterium and was closely related to Haloactinobacterium album YIM 93306(T) with 95.99% similarity. The average nucleotide identity value between strain T3246-1(T) and Haloactinobacterium album YIM 93306(T) was 76.65 %. Based on phenotypic and chemotaxonomic characteristics, strain T3246-1(T) was considered to represent a novel species of the genus Haloactinobacterium, for which the name Haloactinobacterium glacieicola sp. nov. is proposed. The type strain is T3246-1(T) (=CGMCC 1.13535(T)= JCM 32923(T)).